h2dcf da staining Search Results


2 7 dichlorodihydrofluorescein diacetate h2dcf da staining assay  (MedChemExpress)
99
MedChemExpress 2 7 dichlorodihydrofluorescein diacetate h2dcf da staining assay
Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
2 7 Dichlorodihydrofluorescein Diacetate H2dcf Da Staining Assay, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2dcf+da+staining/H2DCFDA/pm40536686-68-0-5
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h2dcf-da  (Becton Dickinson)
90
Becton Dickinson h2dcf-da
Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
H2dcf Da, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2dcf+da+staining/h2dcf+da/pmc09456465-159-7-17
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h2dcf-da - by Bioz Stars, 2026-08
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h2dcf-da 2',7'- dichlorofluorescin diacetate  (Merck KGaA)
90
Merck KGaA h2dcf-da 2',7'- dichlorofluorescin diacetate
Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
H2dcf Da 2',7' Dichlorofluorescin Diacetate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2dcf+da+staining/2%C2%B4+7%C2%B4+dichlorodihydrofluorescein+diacetate++h2dcf+da+/pm40561605-118-3-7
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acetyl ester  (Thermo Fisher)
94
Thermo Fisher acetyl ester
Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
Acetyl Ester, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2dcf+da+staining/Fluorescein+diacetate%2C+97%25%2C+pure/10__35848_slash_1347___4065_slash_acd9b6-67-21-25
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accuritm c6 plus flow cytometer  (Becton Dickinson)
90
Becton Dickinson accuritm c6 plus flow cytometer
Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
Accuritm C6 Plus Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2dcf+da+staining/facscalibur+flow+cytometer/pm35024200-94-18-23
Average 90 stars, based on 1 article reviews
accuritm c6 plus flow cytometer - by Bioz Stars, 2026-08
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2 7 dichlorodihydro fluorescein diacetate  (Thermo Fisher)
99
Thermo Fisher 2 7 dichlorodihydro fluorescein diacetate
Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye <t>H2DCF-DA.</t> Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)
2 7 Dichlorodihydro Fluorescein Diacetate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2dcf+da+staining/Fluorescein/10__1097_slash_fjc__0b013e31815d781d-98-19-22
Average 99 stars, based on 1 article reviews
2 7 dichlorodihydro fluorescein diacetate - by Bioz Stars, 2026-08
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Image Search Results


Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye H2DCF-DA. Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)

Journal: Plant cell reports

Article Title: Myosin XI coordinates ABA-induced stomatal closure via microtubule stability and ROS synthesis in drought-stressed Arabidopsis.

doi: 10.1007/s00299-025-03538-2

Figure Lengend Snippet: Fig. 5 Myosin XI facilitates ABA-induced stomatal closure. A Rep- resentative images of stomata from detached leaves of WT, 2ko, and 3ko after a 2-h treatment with stomatal opening buffer (Mock), fol- lowed by a 30-min treatment with ABA (5 μM) (+ ABA). Scale bar, 25 μm. B Quantification of stomatal apertures in detached leaves of WT, 2ko, and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 30-min treatment with ABA (5 μM) (+ ABA). Data represent mean ± SD (n = 40; ***p < 0.001). C ROS produc- tion in guard cells was detected using the ROS-sensitive fluorescent dye H2DCF-DA. Scale bar, 10 μm. D Quantification of relative fluo- rescence in guard cells of WT, 2ko, and 3ko plants after a 2-h treat- ment with stomatal opening buffer (Mock), followed by a 1-h treat- ment with ABA (30 μM) (+ ABA). Data represent mean ± SD (n = 14; *p < 0.05, **p < 0.01). E cMTs labeled with GFP-TUB6 in guard cells of WT and 3ko were visualized using confocal microscopy. Scale bar, 10 μm. F Quantification of cMTs densities in WT and 3ko following a 2-h treatment with stomatal opening buffer (Mock), then a 1-h treatment with ABA (20 μM) (+ ABA). Data represent mean ± SD (n = 8; *p < 0.05, **p < 0.01)

Article Snippet: 2',7'-dichlorodihydrofluorescein diacetate (H2DCF-DA) staining assay (MedChemExpress, NJ, USA) was employed to visualize H2O2 generation in guard cells, following a previously established method (Zhou et al. 2013).

Techniques: Labeling, Confocal Microscopy